ABSTRACT
BACKGROUNDS: Pityrosporum (P.) ovale is a member of the cutaneous microflora, and is associated with several dermatologic diseases. Little is known about the mechanism by which P. ovale causes these dermatoses, but it has been supposed to be associated with immunologic response. The epidermis is largely composed of keratinocytes, and they not only play an important structural part in forming a physical barrier to microorganisms, but also are important functionally in mediating cutaneous immune reaction by secreting various cytokines. OBJECTIVES: There have been few studies on the interaction between fungi and keratinocytes, especially the effect of P. ovale on the production of cytokines by human keratinocytes. This study is to evaluate the effects of P. ovale on pro-inflammatory cytokines such as interleukin-1alpha (IL-1alpha), tumor necrosis factor-alpha(TNF-alpha), granulocyte-macrophage colony stimulating factor (GM-CSF) producted by human keratinocytes. METHODS: Normal human keratinocytes (NHK) from neonatal foreskin were suspended in culture medium with different concentrations of the P. ovale extract (0.01, 1, 10, 100microgram/ml). The production of IL-1alpha, TNF-alpha and GM-CSF after 24 hours treatment were semiquantitated by RT-PCR. Time-course changes of the cytokines were also observed until 72 hours (0, 6, 12, 24, 48, 72 hours). NHKs were treated with P. ovale culture supernatant (0.1, 0.2, 0.5, 1.0ml) for 24 hours, and the cytokines were measured by RT-PCR and ELISA. RESULTS: IL-1alpha and GM-CSF were increased by treatment with P. ovale extract as a dose-dependent manner. But TNF-alpha production was not affected by the treatement. The level of IL-1alpha was steadily increased with its maximum at 48 hours. The level of GM-CSF was increased for 48 hours and became decreased thereafter. NHKs treated with P. ovale culture supernatant showed the increase of IL-1alpha and GM-CSF synthesis as a dose-dependent manner. CONCLUSIONS: P. ovale induced the pro-inflammatory cytokines in normal keratinocytes, which is supposed to induce inflammatory response by the organism in the skin.
Subject(s)
Humans , Colony-Stimulating Factors , Cytokines , Enzyme-Linked Immunosorbent Assay , Epidermis , Foreskin , Fungi , Granulocyte-Macrophage Colony-Stimulating Factor , Interleukin-1alpha , Keratinocytes , Malassezia , Necrosis , Negotiating , Skin , Skin Diseases , Tumor Necrosis Factor-alphaABSTRACT
Churg-Strauss syndrome is a systemic necrotizing vasculitis occurring in patients with asthma or allergic rhinitis, and characterized by eosinophilic infiltration of various tissues and peripheral eosinophilia. A 28-year-old male was admitted due to painful purpuric papules and patches on both lower legs and both hands with dysesthesia and weakness for 4 days. He had been well until 8 months earlier when asthma developed. Abnormal laboratory findings including peripheral blood eosinophilia, p-ANCA(+), elevated BUN and creatinine, hematuria, proteinuria, and stool occult blood were observed. Parenchymal renal disease of both kidneys was confirmed by abdominal sonogram. Marked muscular atrophy in the first web space of left hand dorsum and difficulty in dorsiflexion of both feet had developed, suggesting mononeuritis multiflex in bilateral peroneal and tibial nerves, left ulnar and radial nerves.
Subject(s)
Adult , Humans , Male , Asthma , Churg-Strauss Syndrome , Creatinine , Eosinophilia , Eosinophils , Foot , Hand , Hematuria , Kidney , Leg , Mononeuropathies , Muscular Atrophy , Occult Blood , Paresthesia , Proteinuria , Radial Nerve , Rhinitis , Tibial Nerve , VasculitisABSTRACT
BACKGROUND: The prevalence and clinical characteristics of dermatomycosis are changed under the various influences such as geographic factor, social environment and development of therapy. OBJECTIVE: The purpose of this study was to evaluate the present status of dermatomycosis and changes on the prevalence, sex and age distribution and causative organisms. METHODS: We performed clinical and mycological studies on 7,568 cases of dermatomycosis among outpatients of Dermatologic clinic of Chonnam University Hospital for 10 years, from January 1991 to December 2000. RESULTS: The incidence of dermatomycosis was 17.6% of out-patients and show the highest incidence in the fourth decades (18.8%). The ratio of male to female was 1.3:1, showing slightly male-predominant pattern. The incidence of Tinea pedis (28.9%) was the highest, followed by Onychomycosis (16.3%), Tinea corporis (11.2%), Tinea cruris (10.1%), Tinea versicolor (9.3%), Tinea manus (8.0%), Candidiasis (6.9%), Tinea faciale (5.5%) and Tinea capitis (3.2%). Coexisting fungal infections were found 846 patients (11.2%) and the cases of Tinea pedis with onychomycosis were the most common. The positive rate of KOH examination was 50.8% and the positive rate of culture on Sabouraud's dextrose agar media was 31.4%. Trichophyton(T.) rubrum was the most common causative organism of dematomycosis (67.7%), followed by Candida albicans (13.0%), Microsporum(M.) canis (9.9%), T. mentagrophytes (7.4%), Epidermophyton floccosum (0.5%), M. gypseum (0.5%), T. verrucosum (0.4%), T. tonsurans (0.3%) and T. violaceum (0.2%). CONCLUSION: Compared with previous studies, the incidence of dermatomycosis increased, especially in females and was evenly distributed throughout all ages. The frequency of tinea pedis and tinea cruris decreased, but increased in onychomycosis.
Subject(s)
Female , Humans , Male , Agar , Age Distribution , Candida albicans , Candidiasis , Dermatomycoses , Epidermophyton , Geography , Glucose , Incidence , Onychomycosis , Outpatients , Prevalence , Social Environment , Tinea , Tinea Capitis , Tinea Pedis , Tinea VersicolorABSTRACT
Lupus panniculitis is a chronic inflammatory disease of subcutaneous fat that occurs in 2~3% of patients with systemic lupus erythematosus. Lupus panniculitis typically presents with firm, indurated subcutaneous nodules or plaques commonly developed in proximal extremities, trunk, head and neck. Breast involvement with lupus panniculitis (lupus mastitis) has been infrequently reported. We experienced a case of lupus mastitis developed in a 44-year-old female confirmed by histologic features and laboratory findings. Antimalarial therapy resulted in the complete resolution of the lesions.
Subject(s)
Adult , Female , Humans , Breast , Extremities , Head , Lupus Erythematosus, Systemic , Mastitis , Neck , Panniculitis, Lupus Erythematosus , Subcutaneous FatABSTRACT
No abstract available.
Subject(s)
Female , Pregnancy , Apoptosis , Hypertension, Pregnancy-Induced , PlacentaABSTRACT
Cyclosporin A(CsA) is now widely used to treat organ transplant recipients. But CsA has various short-and long-term side effects. Especially, gingival hyperplasia is not easy to resolve since its nature is still unknown. This study discusses the pathogenesis of CsA-induced gingival hyperplasia on the basis of data obtained from light and electron microscopic studies of biopsis from patients on CsA treatment after kidney transplantation. Light microscopically, the multilayered squamous epithelium showed an irregular surface of parakeratosis and deep invaginations in the subepithelial tissue. At lamina propria, we observed bundles of irregularly arranged collagen fiber, some fibroblasts, numerous capillary vessels and a large diffuse infiltration of plasma cells. Ultrastructurally, many fibroblasts, collagen fibers, collagen fibrils were present in lamina propria. On the basis of the data collected, we propose that the morphological features of the dimensional increase in gingival tissue associated with CsA treatment in kidney transplant patients may be considered proliferative fibroblasts, collagen fibers, collagen fibrils in lamina propria.
Subject(s)
Humans , Capillaries , Collagen , Cyclosporine , Epithelium , Fibroblasts , Gingival Hyperplasia , Kidney , Kidney Transplantation , Mucous Membrane , Parakeratosis , Plasma Cells , TransplantsABSTRACT
the ineidence of abdominal subcutaneous endometriosis is quite rare we have experienced one case of subcutaneous endometriosis. The typical clinical bistory and local findings of endometriasis enabk us to make the conect diagnosis. the treatment of choice is complete surgical excision of endometrial tissue and post operative medical therapy. This case was reported with a brief review of the comcemed literatures.
Subject(s)
Female , Diagnosis , Endometriosis , Subcutaneous TissueABSTRACT
OBJECTIVE: To investigate the preterm labor & preterm infants care in Kwangju and Chonam. METHODS: A study was conducted for 2,360 newborns who were born at four main hospitals in Kwangju and chonam from January 1, 1995 to December 31, 1997. Data were collected by review of hospital records. RESULTS: 1. The survival rate was 6.90% at 23~34 weeks, 38.60% at 27~28weeks and 90.67% after 31wks. 2. The survival rate divided according to birth weight were 8.89% below 1,000gm, 57.14% in 1,001~1,500gm, 86.75% in 1,501~2,000gm and 93.37% over 2,000gm respectively. 3. Clinical causes of neonatal death were RDS(48.84%) hyperbilirubinemia (20.74%), sepsis(11.95%) and pneumonia(9.67%). 4. Incidence of RDS was 23.22% and it's neonatal mortality was 36.28%. CONCLUSION: The survival rate of preterm birth in Kwang-Ju and Chon-nam was still lower than data from advancing countries, and main cause of neonatal death was respiratory distress syndrome.